Crystal structure of eIF 2B and insights into eIF 2– eIF 2B interactions
نویسندگان
چکیده
منابع مشابه
The association of eIF-2 with Met-tRNAi or eIF-2B alters the specificity of eIF-2 phosphatase.
In unfractioned reticulocyte lysate, interaction of eukaryotic initiation factor 2 (eIF-2) with other components regulates the accessibility of phosphatases and kinases to phosphorylation sites on its alpha and beta subunits. Upon addition of eIF-2 phosphorylated on both alpha and beta subunits (eIF-2(alpha 32P, beta 32P) to lysate, the alpha subunit is rapidly dephosphorylated, but the beta su...
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Protein synthesis initiation factors eIF-1, eIF-4C, eIF4D, and eIF-5 were isolated and purified from rabbit reticulocyte lysates. Crude initiation factors from the high salt ribosomal extract were separated by ammonium sulfate precipitation into Fraction A (0 to 40% saturation) and Fraction BC (40 to 70% saturation). Fraction BC was separated by gel filtration on Sephadex G-100 into two fractio...
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The protein eukaryotic translation initiation factor 5A (eIF-5A) is a highly conserved eukaryotic translation initiation factor (eIF) found in eukaryotes and archaea.1–3 Biochemical and molecular studies revealed that eIF-5A is the sole protein that contains a modified amino acid residue hypusine (Ne-(4-amino-2-hydroxybutyl)lysine).4 The hypusination modification is made by two sequential react...
متن کاملWheat Germ eIF 2 and CoeIF 2
A factor that binds Met-tRNAF’ in a ternary complex (eucaryotic initiation factor (eIF2)) and an auxiliary component that strongly stimulates the activity of the binding factor (CoeIF2) have been purified from extracts of wheat germ. The molecular weights of the two factors, as determined by glycerol gradient centrifugation and sodium dodecyl sulfate gel electrophoresis, are 88,000 and 20,000, ...
متن کاملPurification and properties of eIF-2 phosphatase.
Eukaryotic initiation factor 2 (eIF-2) phosphatase has been purified 840-fold to apparent homogeneity from rabbit reticulocyte lysate. Native eIF-2 phosphatase has a Mr = 98,000, pI = 6.1, s20,w = 5.1, and a Stokes radius = 38 A. A subunit composition of one 60,000-dalton polypeptide and one 38,000-dalton polypeptide is indicated. The Km for [32P]eIF-2 is 30 microM and the Vmax = 1.1 nmol of ph...
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ژورنال
عنوان ژورنال: The FEBS Journal
سال: 2016
ISSN: 1742-464X,1742-4658
DOI: 10.1111/febs.13896